New Step by Step Map For high performance liquid chromatography
New Step by Step Map For high performance liquid chromatography
Blog Article
Two difficulties are inclined to shorten the life span of the analytical column. Initially, solutes that bind irreversibly to the stationary phase degrade the column’s performance by lowering the level of stationary section readily available for effecting a separation. Second, particulate material injected While using the sample may possibly clog the analytical column.
内部にカラムを収納して加熱あるいは冷却を行い、カラムの温度を制御する装置。カラムヒーターとも称する。
Adsorption chromatography involves the interaction of substances Along with the surface area on the stationary stage. A compound’s affinity for that stationary phase establishes its diploma of retention. In reverse-phase HPLC, one example is, nonpolar molecules are held by a polar stationary section.
- 분석결과는 재현성이 우수하며, 특히 오토샘플러 등을 사용함으로써 보다 높은 재현성을 확보할 수 있어 생산성을 한층 더 향상시킬 수 있습니다.
The 3 red circles are binary mobile phases made by combining equal volumes with the pure mobile phases. The ternary cell section proven via the purple circle consists of all a few of the pure cell phases.
Fluoxetine is another title to the antidepressant drug Prozac. The resolve of fluoxetine in serum is a crucial A part of monitoring its therapeutic use.
Preserve a logbook: Document your observations, which include peak designs, retention situations, and any modifications designed to the method. This will help you identify traits and troubleshoot concerns far more properly.
-hydroxybenzoic acid (PH) on the nonpolar C18 column subject into a maximum Evaluation time of 6 min. The shaded spots signify locations wherever a separation is impossible, Using the unresolved solutes identified.
The easiest way to recognize the theoretical and the practical facts talked about in this part is usually to cautiously analyze a typical analytical technique.
The a few crimson circles are binary mobile phases produced by combining equivalent volumes of your pure cellular phases. The ternary mobile section demonstrated with the purple circle consists get more info of all a few in the pure mobile phases.
Conversely, a move amount that is way too small can result in too much band broadening. Test your flow fee configurations and regulate them based on the established approach.
In this particular part we look at the simple plumbing necessary to shift the cellular section with the column and to inject the get more info sample to the mobile stage.
This website page doesn't exist as part of your picked language. Your desire was saved and you may be notified once a page may be viewed inside your language.
Along with the Evaluation system understood, let us handle prevalent troubles that may occur and the way to troubleshoot them.